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Bilaga 3. Publikationer vid IMM 2003-2005 - Karolinska Institutet
2019-10-25 The pTracer™-SV40 vector offers: • SV40 promoter for high-level constitutive expression of your gene of interest • CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the SV40 large T antigen SV40 / hAlb promoter (Liver) in pDRIVE expression plasmid. The albumin gene is transcribed at very high levels in fetal liver and, unlike the adjacent AFP gene, remains active after birth. A small segment of the bovine albumin 5’flanking region, from -170 to +20, is sufficient for promoter activity and specificity . SV40 and α-actin promoters were similar in their expression in muscle tissue, with levels about two-thirds less than that of the CMV promoter. The PgK promoter was the weakest of all five, and gave expression only about one-tenth of the CMV promoter in muscle.
可能包含一个增强子. PGK1 (人/小鼠) 常规表达用. mRNA. 磷酸甘油酸酯激酶基因来源的哺乳动物启动子.
1 The pCMV-Script vector does not contain an ATG initiation codon. A translation initiation sequence must be incorporated if the DNA fragment to A. SV40 promoter가 있는 vector가 따로 존재합니다. 이 경우는 sv40 promoter에 general한 TF가 붙으므로 그 앞쪽에 putative enhancer elements를 cloning하여 transcriptional cofactor들의 function을 찾는 데 필요한 assay입니다.
Risk Evaluation of Potential Environmental Hazards From Low
Aberrant promoter methylation and loss of sup-. av C Annerén — SV40 Tag (semian virus.
IMBIM ANNUAL REPORT 2015 - studyres.com
The majority of the enhancer sequences, however, do not contribute to this activity. SV40 promoter is also fairly strong, though generally somewhat weaker than EF1A and CAGG.
COS-7 and 293T cells). The SV40 promoter is fairly weak in 293T cells compared to the CMV promoter. So for the highest expression, For the highest protein "overexpression",
Addgene: Vector Database - pCS2 .
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hPGK promoter scaffold. (7869) BstBI SV40 polya. hU6. PLCKO.TRC005.sgRNAwStuffer.
Since the promoter region drives transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount of recombinant protein that will be produced. Many common promoters. like CMV, EF1A, and SV40 promoters, are always active and thus referred to as constitutive promoters. Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly-adenylation signal. The 1.8-kb marker is compatible with any expression vector (including Tet System vectors) for generating stable transfectants using CalPhos transfection reagent or a liposome-mediated transfection protocol. SV40 / bAlb promoter (Liver) in pDRIVE expression plasmid. The albumin gene is transcribed at very high levels in fetal liver and, unlike the adjacent AFP gene, remains active after birth.
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The PgK promoter was the weakest of all five, and gave expression only about one-tenth of the CMV promoter in muscle. Cloning into pTracer™-SV40, Continued Cloning into pTracer™-SV40 The graphic below shows the SV40 early enhancer/promoter and the multiple cloning site of pTracer™-SV40. The underlined regions at the 5´ end of the fragment containing the SV40 promoter are the two 72 bp enhancer repeats. Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region drives transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount of recombinant protein that will be produced. Many common promoters. like CMV, EF1A, and SV40 promoters, are always active and thus referred to as constitutive promoters.
The SV40 late promoter, a primary target for T-antigen transcriptional activation, contains a previously described T-antigen-activatable binding site (SV40 nucleotides 186 to 225). Se cree que el SV40 suprime las propiedades transcripcionales del p53, supresor de tumores en humanos, a través del antígeno T SV40 grande y del antígeno T SV40 pequeño.
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Neutrophil gelatinase-associated lipocalin is up-regulated in
Other mechanism: Termination transcription from all polymerase II promoters. The SV40 enhancer. There are factors which inluence gene expression: strength of promoter (consituive or The tPA cDNA was cloned into an SV40-based expression vector. promoter. (magsår ökar risken för magcancer, inflammatorisk tarmsjukdom ökar SV40 – replikering och transformering. SV40-, polyoma- och adenovirus. ( d, höger panel) Figur som visar vikinduktionen av SV40-promotoraktiviteten hos the two p53-response elements, were used to amplify the PRAP1 promoter as The HspA1A promoter-driven luciferase plasmid was gifted by Professor Jianhua The cells were co-transfected with pHspA1A-Luc and pRL-SV40 (a plasmid Construction of human Prdx6 promoter-chloramphenicol acetyltransferase (1) a cell line (SRA01/04) immortalized with SV40, and (2) primary human LECs luciferasreporter pRL-SV40 (Promega, Madison, WI, USA) cotransfekterades som en intern kontroll.
Nr 3 2015 - 16637 Onkologi 3_15
av I Alexandersson · 2015 — promoter strength and plasmid copy number in Saccharomyces cerevisiae. Figur 20: Genkarta över p413ins med GAL1-promotor, SV40 NLS, such as polyomaviruses (SV40), HPVs and members of the herpes virus Sobti, R.C. et al. Aberrant promoter methylation and loss of sup-. av C Annerén — SV40 Tag (semian virus. 40 large antigen).